23 [19-1]:Lip, H&E; This is a low magnification view of the lip. While the outside of the lip is characterized by fully keratinized stratified squamous epithelium (i.e. the epidermis) with hair follicles (large arrow) and sebaceous glands embedded in the dermis, the inside of the lip is lined by a non-keratinized stratified epithelium supported by a lamina propria. [19-2]:Inner lining of the lip, H&E; Higher magnification of the inner lip showing the lining epithelium. [19-3]:Tongue, H&E. Note the skeletal muscle running in three different planes in this cross-section of the tongue. [19-4]:Filiform papillae (arrows), H&E. [19-5]:Fungiform papilla (arrow) H&E. [19-6]:Circumvallate papilla, H&E; Note the many taste buds (small arrows) along the sides of this papilla. A duct (larger arrow) can be seen arising from the serous glands of von Ebner. [19-7]:Circumvallate papilla, H&E; Higher magnification of the moat region around a circumvallate papilla showing taste buds (arrows) and serous glands of van Ebner (S). [19-8]:Taste bud, Iron hematoxylin & aniline blue; Taste bud showing the darker nuclei of the supporting cells, the lighter staining nuclei of the sensory cells (small arrows). The microvilli of these cells project out the taste pore as a taste hairs (large arrow). [19-9]:Parotid gland, H&E; Parts of three lobules can be seen in this low magnification view of the parotid gland. Note the interlobular duct (arrow) seen in the connective tissue septae between the lobules. Can you distinguish this duct from arteries and veins also seen in the connective tissue septae? [19-10]:Parotid Gland, H&E; In the center of the field are sections through pink staining striated ducts. Can you see the striations in the cells lining these ducts? The secretory units stain darker purple due to the RER in their cytoplasm. A small intercalated duct can also be seen (arrow). [19-11]:Parotid Gland, H&E; This is a parotid gland from an older individual which has accumulated numerous fat cells. Do not confuse the leached out fat droplets for the more tubular mucin producing glandular units seen in the submandibular and lingual glands. [19-12]:Submandibular Gland, H&E; Most of the secretory units are serous mixed in with a small number of mucous secretory units(these appear more leached out). [19-13]:Submandibular Gland, H&E; Note the serous demilunes (arrows) capping the mucous secretory units. [19-14]:Sublingual Gland, H&E; Most of the secretory units are mucous mixed in with a small number of serous secretory units. [19-15]:Schematic diagram of a tooth. [19-16]:X-ray of teeth. In this x-ray try to distinguish the thin layer of enamel from the grayer dentin. Also distinguish the pulp cavity and elements of the alveolar bone. [19-17]:Tooth Crown, Fuchsin; The brown lines seen in the enamel (e) represents the lines of Retzius. d; dentin. p; region of the pulp cavity. [19-18]:Tooth, H&E; In this histological section of the tooth, the enamal has been lost due to decalcification. Note the clear pulp cavity in the dentin (d). Note also the periodontal ligament (arrows) situated between the alveolar bone (a) and the dark, thin layer of cementum surrounding the dentin. [19-19]:Tooth, H&E;Vicinity of the gingival sulcus (arrow) Note the sulcus is lined by a non-keratinized stratified squamous epithelium while the outside of the gingiva is lined by a fully keratinized stratified squamous epithelium. [19-20]:Tooth, H&E; Vicinity of the root of the tooth showing elements of the periodontal ligament (p) running between the alveolar bone (a) and the cementum (c). d; dentin. [19-21]:Tooth, Trichrome stain; Elements of the periodontal ligament (p) can be seen embedding in the cementum (c) as Sharpey’s fibers (arrows). Can you make out section through dentinal tubules in the dentin (d)? [19-22]:Developing tooth, H&E; Low magnification view of the developing tooth. The developing pulp cavity (p) is lined by an odontoblastic layer of cells (small arrows) which is actively making dentin (d). The region of the large arrow is where enamel has been leached out as a result of decalcifying the tooth. [19-23]:Developing tooth, H&E; Higher magnification of the developing tooth seen in the previous slide. Odontoblasts (o) can be seen giving rise to predentin (small arrow) which will become more calcified to form the mature dentin (d). Some of the enamal which was produced by the ameloblasts (a) remains and can be detected despite decalcification of the tooth (large arrow).